pd l2 primary antibodies  (Proteintech)

Journal: Journal of Cancer

Article Title: Expression of Programmed Death Ligand-2 is associated with Prognosis in Nasopharyngeal Carcinoma Microenviroment

doi: 10.7150/jca.77643

Figure Lengend Snippet: Expression of PD-L2 in NPC tumor and stoma tissues. A and B Weak PD-L2 staining in NPC tumor tissues of TMA samples under low and high magnifications; C and D Strong PD-L2 staining in tumor tissues is shown under low and high magnifications; E and F Weak PD-L2 staining in NPC stoma tissues of TMA samples under low and high magnifications; G and H Strong PD-L2 staining in stroma tissues is shown under low and high magnifications.

Article Snippet: Primary antibodies against PD-L2 (1:500 dilution, ab214221, Abcam, USA), CD4 (1:500 dilution, ab183685, Abcam, USA), Foxp3 (1:200 dilution, AF3240, USA), and CD68 (1:500 dilution, ABIN370601, Dako, Denmark) were used in this study.

Techniques: Expressing, Staining

Journal: Journal of Cancer

Article Title: Expression of Programmed Death Ligand-2 is associated with Prognosis in Nasopharyngeal Carcinoma Microenviroment

doi: 10.7150/jca.77643

Figure Lengend Snippet: Selection of the cutoff score. Receiver operating characteristic curve analysis was employed to determine the cutoff score for the designation of “high expression” of PD-L2. The sensitivity and specificity for each outcome of PD-L2 membranous staining of stromal tissues were plotted: A T classification (AUC=0.607, P =0.001); B N classification (AUC=0.571, P =0.120); C Clinical stage (AUC=0.612, P =0.002); D Cancer progression (AUC=0.610, P=0.001); E Survival status (AUC=0.618, P=0.001).

Article Snippet: Primary antibodies against PD-L2 (1:500 dilution, ab214221, Abcam, USA), CD4 (1:500 dilution, ab183685, Abcam, USA), Foxp3 (1:200 dilution, AF3240, USA), and CD68 (1:500 dilution, ABIN370601, Dako, Denmark) were used in this study.

Techniques: Selection, Expressing, Staining

Journal: Journal of Cancer

Article Title: Expression of Programmed Death Ligand-2 is associated with Prognosis in Nasopharyngeal Carcinoma Microenviroment

doi: 10.7150/jca.77643

Figure Lengend Snippet: The corresponding cutoff score of PD-L2 stroma and tumor expression for each clinicopathological feature according to ROC curve analysis

Article Snippet: Primary antibodies against PD-L2 (1:500 dilution, ab214221, Abcam, USA), CD4 (1:500 dilution, ab183685, Abcam, USA), Foxp3 (1:200 dilution, AF3240, USA), and CD68 (1:500 dilution, ABIN370601, Dako, Denmark) were used in this study.

Techniques: Expressing

Journal: Journal of Cancer

Article Title: Expression of Programmed Death Ligand-2 is associated with Prognosis in Nasopharyngeal Carcinoma Microenviroment

doi: 10.7150/jca.77643

Figure Lengend Snippet: Association of PD-L2 expression and clinicopathological characteristics in NPC patients

Article Snippet: Primary antibodies against PD-L2 (1:500 dilution, ab214221, Abcam, USA), CD4 (1:500 dilution, ab183685, Abcam, USA), Foxp3 (1:200 dilution, AF3240, USA), and CD68 (1:500 dilution, ABIN370601, Dako, Denmark) were used in this study.

Techniques: Expressing, Staining

Journal: Journal of Cancer

Article Title: Expression of Programmed Death Ligand-2 is associated with Prognosis in Nasopharyngeal Carcinoma Microenviroment

doi: 10.7150/jca.77643

Figure Lengend Snippet: Univariate Cox proportional hazards regression analysis for PD-L2

Article Snippet: Primary antibodies against PD-L2 (1:500 dilution, ab214221, Abcam, USA), CD4 (1:500 dilution, ab183685, Abcam, USA), Foxp3 (1:200 dilution, AF3240, USA), and CD68 (1:500 dilution, ABIN370601, Dako, Denmark) were used in this study.

Techniques: Expressing

Journal: Journal of Cancer

Article Title: Expression of Programmed Death Ligand-2 is associated with Prognosis in Nasopharyngeal Carcinoma Microenviroment

doi: 10.7150/jca.77643

Figure Lengend Snippet: Association between PD-L2 expression and NPC patient survival. TMA analyses were conducted in a cohort of 557 NPC patients diagnosed at M0. A The five-year overall survival (OS) rate was 68.7%. B The five-year disease-free survival (DFS) rate was 62.6%. C and D High PD-L2 expression levels in stromal tissues were significantly associated with OS (P = 0.002) and disease-free survival (P = 0.001) in all NPC patients. F High PD-L2 expression levels in tumor tissues were significantly associated with disease-free survival (P = 0.048), and there were no significant differences in five-year OS (P = 0.134) in all NPC patients (E) .

Article Snippet: Primary antibodies against PD-L2 (1:500 dilution, ab214221, Abcam, USA), CD4 (1:500 dilution, ab183685, Abcam, USA), Foxp3 (1:200 dilution, AF3240, USA), and CD68 (1:500 dilution, ABIN370601, Dako, Denmark) were used in this study.

Techniques: Expressing

Journal: Journal of Cancer

Article Title: Expression of Programmed Death Ligand-2 is associated with Prognosis in Nasopharyngeal Carcinoma Microenviroment

doi: 10.7150/jca.77643

Figure Lengend Snippet: Association between PD-L2 expression and NPC patient survival at different stages. A and B High PD-L2 expression in the stroma was significantly associated with OS (P < 0.001) and disease-free survival (P = 0.001) in NPC patients at early stages (I-II). C and D No significant differences in five-year OS and DFS rates were found between groups with low and high expression of PD-L2 in tumor tissues of NPC patients at early stages (I-II). E and F No significant differences in five-year OS and DFS rates were found between groups with low and high expression of PD-L2 in NPC patients at late stages (III-IV). H High PD-L2 expression levels in tumor tissues were significantly associated with disease-free survival (P = 0.025), and there were no significant differences in five-year OS in all NPC patients (G) .

Article Snippet: Primary antibodies against PD-L2 (1:500 dilution, ab214221, Abcam, USA), CD4 (1:500 dilution, ab183685, Abcam, USA), Foxp3 (1:200 dilution, AF3240, USA), and CD68 (1:500 dilution, ABIN370601, Dako, Denmark) were used in this study.

Techniques: Expressing

Journal: Journal of Cancer

Article Title: Expression of Programmed Death Ligand-2 is associated with Prognosis in Nasopharyngeal Carcinoma Microenviroment

doi: 10.7150/jca.77643

Figure Lengend Snippet: Multivariate Cox proportional hazards regression analysis for PD-L2

Article Snippet: Primary antibodies against PD-L2 (1:500 dilution, ab214221, Abcam, USA), CD4 (1:500 dilution, ab183685, Abcam, USA), Foxp3 (1:200 dilution, AF3240, USA), and CD68 (1:500 dilution, ABIN370601, Dako, Denmark) were used in this study.

Techniques: Expressing

Journal: Journal of Cancer

Article Title: Expression of Programmed Death Ligand-2 is associated with Prognosis in Nasopharyngeal Carcinoma Microenviroment

doi: 10.7150/jca.77643

Figure Lengend Snippet: Association between PD-L2 molecular expression and immune cell density

Article Snippet: Primary antibodies against PD-L2 (1:500 dilution, ab214221, Abcam, USA), CD4 (1:500 dilution, ab183685, Abcam, USA), Foxp3 (1:200 dilution, AF3240, USA), and CD68 (1:500 dilution, ABIN370601, Dako, Denmark) were used in this study.

Techniques: Expressing

Journal: Bioengineered

Article Title: Exosomes loaded with programmed death ligand-1 promote tumor growth by immunosuppression in osteosarcoma

doi: 10.1080/21655979.2021.1996509

Figure Lengend Snippet: The expression level of PD-L1 in MG63, HOS/MNNG, Saos-2, SJSA-1, U2OS, SW1353, 143B, T1-73, KHOS/NP, and hFOB 1.19 cells was determined by Western blotting assay

Article Snippet: Then, the protein-loaded PVDF membrane was mixed with 5% skim milk to block nonspecific binding proteins and incubated with a solution of primary antibodies against PD-L1 (1:800, R&D, Minnesota, USA), PD-L2 (1:800, R&D, Minnesota, USA), GRP 94 (1:800, R&D, Minnesota, USA), HSP70 (1:800, R&D, Minnesota, USA), HSP90 (1:800, R&D, Minnesota, USA), CD9 (1:800, R&D, Minnesota, USA), CD81 (1:800, R&D, Minnesota, USA), PD-1 (1:800, R&D, Minnesota, USA), and GADPH (1:800, R&D, Minnesota, USA) [ ].

Techniques: Expressing, Western Blot

Journal: Bioengineered

Article Title: Exosomes loaded with programmed death ligand-1 promote tumor growth by immunosuppression in osteosarcoma

doi: 10.1080/21655979.2021.1996509

Figure Lengend Snippet: Exosomes were extracted using ultracentrifugation. a. Nanoparticle tracking analysis was used to measure the distribution of particle size (60,000×). b. The ultrastructure of exosomes was visualized by TEM. c. The expression level of HSP70, HSP90, CD63, CD81, PD-1, PD-L1, PD-L2, GRP 94 and GAPDH was detected by Western blotting assay

Article Snippet: Then, the protein-loaded PVDF membrane was mixed with 5% skim milk to block nonspecific binding proteins and incubated with a solution of primary antibodies against PD-L1 (1:800, R&D, Minnesota, USA), PD-L2 (1:800, R&D, Minnesota, USA), GRP 94 (1:800, R&D, Minnesota, USA), HSP70 (1:800, R&D, Minnesota, USA), HSP90 (1:800, R&D, Minnesota, USA), CD9 (1:800, R&D, Minnesota, USA), CD81 (1:800, R&D, Minnesota, USA), PD-1 (1:800, R&D, Minnesota, USA), and GADPH (1:800, R&D, Minnesota, USA) [ ].

Techniques: Expressing, Western Blot

Journal: Bioengineered

Article Title: Exosomes loaded with programmed death ligand-1 promote tumor growth by immunosuppression in osteosarcoma

doi: 10.1080/21655979.2021.1996509

Figure Lengend Snippet: Exosomes extracted from OS cells inactivated Jurkat cells through its loaded PD-L1. a. The production of IFN-γ was determined in MG63 Exosome, Saos-2 Exsome, and hFOB1.19 Exosome by ELISA assay (p < 0.05, p < 0.01). b. Western blotting assay was used to evaluate the expression level of PD-L1 in Jurkat cells treated with PBS, siNC, siPD-L1#1, and siPD-L1#2. c. The secretion of IFN-γ from MG63 cells with or without Exosome and PMA+ionomycn treatment was measured by ELISA assay (p < 0.01)

Article Snippet: Then, the protein-loaded PVDF membrane was mixed with 5% skim milk to block nonspecific binding proteins and incubated with a solution of primary antibodies against PD-L1 (1:800, R&D, Minnesota, USA), PD-L2 (1:800, R&D, Minnesota, USA), GRP 94 (1:800, R&D, Minnesota, USA), HSP70 (1:800, R&D, Minnesota, USA), HSP90 (1:800, R&D, Minnesota, USA), CD9 (1:800, R&D, Minnesota, USA), CD81 (1:800, R&D, Minnesota, USA), PD-1 (1:800, R&D, Minnesota, USA), and GADPH (1:800, R&D, Minnesota, USA) [ ].

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Expressing

Journal: Bioengineered

Article Title: Exosomes loaded with programmed death ligand-1 promote tumor growth by immunosuppression in osteosarcoma

doi: 10.1080/21655979.2021.1996509

Figure Lengend Snippet: PD-L1 induced the growth of OS cells. a. The expression level of HSP70, HSP90, CD9, PD-L1, GRP 94 and GAPDH was determined by Western blotting. b. Cell viability was measured by CCK-8 assay. c-d. The in vivo growth of OS cells was evaluated by xenograft model (p < 0.01). e. Flow cytometry was used to determine the proportion of CD3 + T cells in the tumor tissues (p < 0.01)

Article Snippet: Then, the protein-loaded PVDF membrane was mixed with 5% skim milk to block nonspecific binding proteins and incubated with a solution of primary antibodies against PD-L1 (1:800, R&D, Minnesota, USA), PD-L2 (1:800, R&D, Minnesota, USA), GRP 94 (1:800, R&D, Minnesota, USA), HSP70 (1:800, R&D, Minnesota, USA), HSP90 (1:800, R&D, Minnesota, USA), CD9 (1:800, R&D, Minnesota, USA), CD81 (1:800, R&D, Minnesota, USA), PD-1 (1:800, R&D, Minnesota, USA), and GADPH (1:800, R&D, Minnesota, USA) [ ].

Techniques: Expressing, Western Blot, CCK-8 Assay, In Vivo, Flow Cytometry

Journal: Bioengineered

Article Title: Exosomes loaded with programmed death ligand-1 promote tumor growth by immunosuppression in osteosarcoma

doi: 10.1080/21655979.2021.1996509

Figure Lengend Snippet: Exosomal PD-L1 induced the growth of OS cells. a-b. The in vivo growth of OS cells was evaluated by xenograft model (p < 0.01). c. Flow cytometry was used to determine the proportion of CD3 + T cells in the tumor tissues (p < 0.05)

Article Snippet: Then, the protein-loaded PVDF membrane was mixed with 5% skim milk to block nonspecific binding proteins and incubated with a solution of primary antibodies against PD-L1 (1:800, R&D, Minnesota, USA), PD-L2 (1:800, R&D, Minnesota, USA), GRP 94 (1:800, R&D, Minnesota, USA), HSP70 (1:800, R&D, Minnesota, USA), HSP90 (1:800, R&D, Minnesota, USA), CD9 (1:800, R&D, Minnesota, USA), CD81 (1:800, R&D, Minnesota, USA), PD-1 (1:800, R&D, Minnesota, USA), and GADPH (1:800, R&D, Minnesota, USA) [ ].

Techniques: In Vivo, Flow Cytometry

Journal: Bioengineered

Article Title: Exosomes loaded with programmed death ligand-1 promote tumor growth by immunosuppression in osteosarcoma

doi: 10.1080/21655979.2021.1996509

Figure Lengend Snippet: Exosomes extracted from PD-L1 positive OS tissues inactivated Jurkat cells. a. Immunohistochemical assay was used to determine the expression level of PD-L1 in the clinical tumor tissues. The Bar length was 25 μm. b. Relationship between the amount of PD-L1-positive exosomes and the expression of PD-L1 in tumor tissues. c. The production of IFN-γ was determined by ELISA assay (p < 0.01)

Article Snippet: Then, the protein-loaded PVDF membrane was mixed with 5% skim milk to block nonspecific binding proteins and incubated with a solution of primary antibodies against PD-L1 (1:800, R&D, Minnesota, USA), PD-L2 (1:800, R&D, Minnesota, USA), GRP 94 (1:800, R&D, Minnesota, USA), HSP70 (1:800, R&D, Minnesota, USA), HSP90 (1:800, R&D, Minnesota, USA), CD9 (1:800, R&D, Minnesota, USA), CD81 (1:800, R&D, Minnesota, USA), PD-1 (1:800, R&D, Minnesota, USA), and GADPH (1:800, R&D, Minnesota, USA) [ ].

Techniques: Immunohistochemical staining, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Molecular cancer therapeutics

Article Title: A correlative analysis of PD-L1, PD-1, PD-L2, EGFR, HER2, and HER3 expression in oropharyngeal squamous cell carcinoma

doi: 10.1158/1535-7163.MCT-17-0504

Figure Lengend Snippet: Patient Characteristics and Tumor Staining

Article Snippet: Formalin-fixed, paraffin-embedded tissue sections were used for immunohistochemistry (IHC), as described previously. ( 13 ) The slides were incubated with primary antibodies for PD-L1 (Cell Signaling Technology, dilution 1:20), PD-L2 (Cell Signaling Technology, dilution 1:50), PD-1 (Abcam, dilution 1:100), EGFR (Biogenex, dilution 1:200), HER2 (Cell Signaling Technology, dilution 1:200), or HER3 (Cell Signaling Technology, dilution 1:100).

Techniques:

Journal: Molecular cancer therapeutics

Article Title: A correlative analysis of PD-L1, PD-1, PD-L2, EGFR, HER2, and HER3 expression in oropharyngeal squamous cell carcinoma

doi: 10.1158/1535-7163.MCT-17-0504

Figure Lengend Snippet: Univariate Association with p16

Article Snippet: Formalin-fixed, paraffin-embedded tissue sections were used for immunohistochemistry (IHC), as described previously. ( 13 ) The slides were incubated with primary antibodies for PD-L1 (Cell Signaling Technology, dilution 1:20), PD-L2 (Cell Signaling Technology, dilution 1:50), PD-1 (Abcam, dilution 1:100), EGFR (Biogenex, dilution 1:200), HER2 (Cell Signaling Technology, dilution 1:200), or HER3 (Cell Signaling Technology, dilution 1:100).

Techniques:

Journal: Molecular cancer therapeutics

Article Title: A correlative analysis of PD-L1, PD-1, PD-L2, EGFR, HER2, and HER3 expression in oropharyngeal squamous cell carcinoma

doi: 10.1158/1535-7163.MCT-17-0504

Figure Lengend Snippet: Univariate Association of EGFR, HER2, HER3 and PD-1, PD-L1, PD-L2

Article Snippet: Formalin-fixed, paraffin-embedded tissue sections were used for immunohistochemistry (IHC), as described previously. ( 13 ) The slides were incubated with primary antibodies for PD-L1 (Cell Signaling Technology, dilution 1:20), PD-L2 (Cell Signaling Technology, dilution 1:50), PD-1 (Abcam, dilution 1:100), EGFR (Biogenex, dilution 1:200), HER2 (Cell Signaling Technology, dilution 1:200), or HER3 (Cell Signaling Technology, dilution 1:100).

Techniques:

Journal: Molecular cancer therapeutics

Article Title: A correlative analysis of PD-L1, PD-1, PD-L2, EGFR, HER2, and HER3 expression in oropharyngeal squamous cell carcinoma

doi: 10.1158/1535-7163.MCT-17-0504

Figure Lengend Snippet: Multivariate Analysis of Advanced Nodal Metastasis

Article Snippet: Formalin-fixed, paraffin-embedded tissue sections were used for immunohistochemistry (IHC), as described previously. ( 13 ) The slides were incubated with primary antibodies for PD-L1 (Cell Signaling Technology, dilution 1:20), PD-L2 (Cell Signaling Technology, dilution 1:50), PD-1 (Abcam, dilution 1:100), EGFR (Biogenex, dilution 1:200), HER2 (Cell Signaling Technology, dilution 1:200), or HER3 (Cell Signaling Technology, dilution 1:100).

Techniques:

Journal: Molecular cancer therapeutics

Article Title: A correlative analysis of PD-L1, PD-1, PD-L2, EGFR, HER2, and HER3 expression in oropharyngeal squamous cell carcinoma

doi: 10.1158/1535-7163.MCT-17-0504

Figure Lengend Snippet: Multivariate Survival Analysis

Article Snippet: Formalin-fixed, paraffin-embedded tissue sections were used for immunohistochemistry (IHC), as described previously. ( 13 ) The slides were incubated with primary antibodies for PD-L1 (Cell Signaling Technology, dilution 1:20), PD-L2 (Cell Signaling Technology, dilution 1:50), PD-1 (Abcam, dilution 1:100), EGFR (Biogenex, dilution 1:200), HER2 (Cell Signaling Technology, dilution 1:200), or HER3 (Cell Signaling Technology, dilution 1:100).

Techniques: